What do you think of ELISA testing for ANA as opposed to IFA testing?

Question

What do you think of ELISA testing for ANA as opposed to IFA testing?

Answer

For those not familiar, many if not all of the larger commercial labs have switched theier ANA testing to ELISA systems.  In my expereince there is a difference between the older IFA (immunofluoresce) testing and ELISA– some patients are positive only on one of the assays.  I could see how ELISA might be more reproducible, but I would still favor IFA.  The future will likely be all ELISA.

Alan Matsumoto, M.D.

About Alan Matsumoto, M.D.

Part-time Faculty, Division of Rheumatology
Johns Hopkins University